Search Results for: lncrna
Postdoc Position Available – Investigating the role of lncRNAs in autoimmune diseases
University Medical Center Groningen
Department: Genetics
Work location: Groningen
Apply no later than:02 February 2014
Working environment
The Department of Genetics at the University Medical Center Groningen, the Netherlands, is an ambitious, dynamic, and international environment, with state-of-the-art facilities. The research section is located in the European Research Institute for the Biology of Aging (ERIBA) together with other departments that all share a common interest in applying systems biological approaches to the study of the genetic factors involved in human disease and aging, in human cells and in model organisms. The Department of Genetics collaborates widely with other groups in Groningen and at a national and international level.
Links: www.geneticsgroningen.nl or www.systemsgenetics.nl
Job description
Prof. Cisca Wijmenga has discovered auto-immune disease-associated SNPs that affect expression of protein-encoding and long non-coding RNA genes. You will investigate the biological processes in which these SNPs are involved using state-of-the-art functional genomics approaches (e.g. groSeq, ChIPseq, 3Cseq, shRNA technology, iPS cells, genome editing technology (e.g. CRISPR/CAS system)). You will be part of a team of three PhD students and two postdocs with backgrounds in statistical genetics, molecular biology, bioinformatics, and immunology. You are aiming to start up your own research line.
Incoming search terms:
- long non-coding RNAs Environmental factors
lncRNAs and Epigenetic Modulations: Potential Hotspots
from Genetic Engineering News
Market and Technology Analysis
The focus of this GEN Market & Tech Analysis report is to present a snapshot of regional epigenetic down- and up-regulation in cancer with a focus toward prostate cancer.
We present here long-range epigenetic silencing (LRES) as a means for downregulation of stretches of the genome as well as long-range epigenetic activation (LREA). These two opposing effects appear to be mediated by an interplay between DNA methylation and chromatin modification. We’ve focused this analysis on an interrogation of prostate cancer.
Incoming search terms:
- harvard elife lncrnas
- metastasis as a therapeutic target for Cancer treatment
H19 lncRNA controls gene expression of the Imprinted Gene Network by recruiting MBD1
The H19 gene controls the expression of several genes within the Imprinted Gene Network (IGN), involved in growth control of the embryo. However, the underlying mechanisms of this control remain elusive. Here, researchers from the CNRS, France identified the methyl-CpG–binding domain protein 1 MBD1 as a physical and functional partner of the H19 long noncoding RNA (lncRNA). The H19 lncRNA–MBD1 complex is required for the control of five genes of the IGN. For three of these genes—Igf2 (insulin-like growth factor 2), Slc38a4 (solute carrier family 38 member 4), and Peg1 (paternally expressed gene 1)—both MBD1 and H3K9me3 binding were detected on their differentially methylated regions. The H19 lncRNA–MBD1 complex, through its interaction with histone lysine methyltransferases, therefore acts by bringing repressive histone marks on the differentially methylated regions of these three direct targets of the H19 gene. This data suggest that, besides the differential DNA methylation found on the differentially methylated regions of imprinted genes, an additional fine tuning of the expressed allele is achieved by a modulation of the H3K9me3 marks, mediated by the association of the H19 lncRNA with chromatin-modifying complexes, such as MBD1. This results in a precise control of the level of expression of growth factors in the embryo.
- Monnier P, Martinet C, Pontis J, Stancheva I, Ait-Si-Ali S, Dandolo L. (2013) H19 lncRNA controls gene expression of the Imprinted Gene Network by recruiting MBD1. Proc Natl Acad Sci U S A [Epub ahead of print]. [abstract]
Incoming search terms:
- Monnier P Martinet C Pontis J Stancheva I Ait-Si-Ali S Dandolo L (2013) H19 lncRNA controls gene expression of the Imprinted Gene Network by recruiting MBD1 Proc Natl Acad Sci U S A [Epub ahead of print]
PhD Position in Computational Biology of lncRNAs: Aachen, Germany
Location:
Posted: October 28, 2023
Expires: December 21, 2023
The Computational Biology Group of the Interdisciplinary Center for Clinical Research (IZKF) Aachen, RWTH Aachen University Hospital, Aachen, invites applicants for a PhD candidate in computational biology methods for modeling and detection of regulatory roles of long non-coding RNA during cell differentiation.The project is based on the development of computational methods to predict functional sites of long non-coding RNAs (lncRNA) from their sequence. In particular, we are interested in interaction of lncRNA with histone modifying proteins and DNA. Functional sites will be derived from the analysis of high-throughput methods such as RIP, CHART, CHIRP, and ChIP sequencing. This project will be performed in collaboration with stem cell specialists from the Institute for Biomedical Engineering, RWTH Aachen University.Applicants should hold a M.Sc. in Bioinformatics, Computer Science or related areas. Experience in the analysis of biological sequences and gene regulation is desirable. The candidate should have solid programming skills (C, Python and R) and acquaintance with Linux. Experience with high performance computing is a plus. The working language of the group is English.The position is based on the German TV-L 13 salary scale, including all German social benefits (health insurance and pension scheme). The expected starting date is March 2014. Interested candidates should send a brief statement of research interests, CV and the names of three references to jobs@costalab.org.The Computational Biology Group is supported by the Interdisciplinary Center for Clinical Research Aachen (IZKF) and hosted by the Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Hospital and the Helmholtz Institute for Biomedical Engineering, RWTH Aachen.
LncRNAs Expression in Preeclampsia Placenta
Long non-coding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. They are aberrantly expressed in many types of diseases. In this study, we aimed to investigate the lncRNA profiles in preeclampsia. Preeclampsia has been observed in patients with molar pregnancy where a fetus is absent, which demonstrate that the placenta is sufficient to cause this condition.
In this study, researchers at the Jiangxi Maternal and Child Health Hospital, China described the lncRNA profiles in six preeclampsia placentas (T) and five normal pregnancy placentas (N) using microarray. With abundant and varied probes accounting for 33,045 LncRNAs in their microarray, 28,443 lncRNAs that were expressed at a specific level were detected. From the data, they found 738 lncRNAs that were differentially expressed (≥1.5-fold-change) among preeclampsia placentas compared with controls. Coding-non-coding gene co-expression networks (CNC network) were constructed based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. According to the CNC network and GO analysis of differentially expressed lncRNAs/mRNAs, they selected three lncRNAs to analyze the relationship between lncRNAs and preeclampsia. LOC391533, LOC284100, and CEACAMP8 were evaluated using qPCR in 40 preeclampsia placentas and 40 controls. These results revealed that three lncRNAs were aberrantly expressed in preeclampsia placentas compared with controls.
This study is the first study to determine the genome-wide lncRNAs expression patterns in preeclampsia placenta using microarray. These results revealed that clusters of lncRNAs were aberrantly expressed in preeclampsia placenta compared with controls, which indicated that lncRNAs differentially expressed in preeclampsia placenta might play a partial or key role in preeclampsia development. Misregulation of LOC391533, LOC284100, and CEACAMP8 might contribute to the mechanism underlying preeclampsia. Taken together, this study may provide potential targets for the future treatment of preeclampsia and novel insights into preeclampsia biology.
- He X, He Y, Xi B, Zheng J, Zeng X, et al. (2013) LncRNAs Expression in Preeclampsia Placenta Reveals the Potential Role of LncRNAs Contributing to Preeclampsia Pathogenesis. PLoS ONE 8(11), e81437. [article]