Search Results for: long non coding rna discovery
Genome-wide discovery and characterization of maize long non-coding RNAs.
Long non-coding RNAs (lncRNAs) are transcripts that are 200 bp or longer, do not encode proteins, and potentially play important roles in eukaryotic gene regulation. However, the number, characteristics and expression inheritance pattern of lncRNAs in maize are still largely unknown.
By exploiting available public EST databases, maize whole genome sequence annotation and RNA-seq datasets from 30 different experiments, a team led by researchers at the University of Minnesota identified 20,163 putative lncRNAs. Of these lncRNAs, more than 90% are predicted to be the precursors of small RNAs, while 1,704 are considered to be high-confidence lncRNAs. High confidence lncRNAs have an average transcript length of 463 bp and genes encoding them contain fewer exons than annotated genes. By analyzing the expression pattern of these lncRNAs in 13 distinct tissues and 105 maize recombinant inbred lines, they show that more than 50% of the high confidence lncRNAs are expressed in a tissue-specific manner, a result that is supported by epigenetic marks. Intriguingly, the inheritance of lncRNA expression patterns in 105 recombinant inbred lines reveals apparent transgressive segregation, and maize lncRNAs are less affected by cis- than by trans- genetic factors.
- Li L, Eichten SR, Shimizu R, Petsch K, Yeh CT, Wu W, Chettoor AM, Givan SA, Cole RA, Fowler JE, Evans MM, Scanlon MJ, Yu J, Schnable PS, Timmermans MC, Springer NM, Muehlbauer GJ. (2014) Genome-wide discovery and characterization of maize long non-coding RNAs. Genome Biol [Epub ahead of print]. [abstract]
Long Non-coding RNAs Can Encode Proteins After All
Case Western Reserve Investigators Discover Novel Cellular Genes by Uncovering Uncharacterized RNAs that Encode Proteins
Case Western Reserve School of Medicine scientists have made an extraordinary double discovery. First, they have identified thousands of novel long non-coding ribonucleic acid (lncRNA) transcripts. Second, they have learned that some of them defy conventional wisdom regarding lncRNA transcripts, because they actually do direct the synthesis of proteins in cells.
Both of the breakthroughs are detailed in the June 12 issue of Cell Reports.
Kristian E. Baker, PhD, assistant professor in the Center for RNA Molecular Biology, led the team that applied high throughput gene expression analysis to yield these impressive findings, which ultimately could lead to treatments for cancer and some genetic disorders.
RNA regulatory networks in animals and plants: a long noncoding RNA perspective
A recent highlight of genomics research has been the discovery of many families of transcripts which have function but do not code for proteins. An important group is long noncoding RNAs (lncRNAs), which are typically longer than 200 nt, and whose members originate from thousands of loci across genomes. The authors review progress in understanding the biogenesis and regulatory mechanisms of lncRNAs. They describe diverse computational and high throughput technologies for identifying and studying lncRNAs. They discuss the current knowledge of functional elements embedded in lncRNAs as well as insights into the lncRNA-based regulatory network in animals. The authors also describe genome-wide studies of large amount of lncRNAs in plants, as well as knowledge of selected plant lncRNAs with a focus on biotic/abiotic stress-responsive lncRNAs.
- Bai Y, Dai X, Harrison AP, Chen M. (2014) RNA regulatory networks in animals and plants: a long noncoding RNA perspective. Brief Funct Genomics [Epub ahead of print]. [abstract]
Incoming search terms:
- Long non coding RNA discovery
Role of the lncRNA-p53 regulatory network in cancer
Advances in functional genomics have led to discovery of a large group of previous uncharacterized long non-coding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs may serve as master gene regulators through various mechanisms. Dysregulation of lncRNAs is often associated with a variety of human diseases including cancer. Of significant interest, recent studies suggest that lncRNAs participate in the p53 tumor suppressor regulatory network. In this review, the authors discuss how lncRNAs serve as p53 regulators or p53 effectors. Further characterization of these p53-associated lncRNAs in cancer will provide a better understanding of lncRNA-mediated gene regulation in the p53 pathway. As a result, lncRNAs may prove to be valuable biomarkers for cancer diagnosis or potential targets for cancer therapy.
- Zhang A, Xu M, Mo YY. (2014) Role of the lncRNA-p53 regulatory network in cancer. J Mol Cell Biol [Epub ahead of print]. [article]
Incoming search terms:
- h19 dysregulation